Large scale zigzag pattern emerging from circulating active shakers

We report the emergence of large zigzag bands in a population of reversibly actuated magnetic rotors that behave as active shakers, namely squirmers that shake the fluid around them without moving. The shakers collectively organize into dynamic structures displaying self-similar growth and generate topological defects in the form of cusps that connect vortices of rolling particles with alternating chirality. By combining experimental analysis with particle-based simulation, we show that the special flow field created by the shakers is the only ingredient needed to reproduce the observed spatiotemporal pattern. We unveil a self-organization scenario in a collection of driven particles in a viscoelastic medium emerging from the reduced particle degrees of freedom, as here the frozen orientational motion of the shakers

Retraction of thin films coated by insoluble surfactants

We investigate the retraction of a circular thin film coated with insoluble surfactants, which is punctured at its centre. We assume that the surface pressure of the liquid-gas interface is related to the number density of surfactants through a linear equation of state, which is characterized by a single parameter: the Gibbs dilation modulus. To solve the governing equations and track the deformation of the domain, we use the finite element method with an arbitrary Lagrangian-Eulerian approach where the film surface is sharp. Our simulations show that the surface elasticity introduced by the surfactants slows down the retraction and introduces oscillations at early times. In agreement with previous experiments and theoretical analysis, we find that the presence of surfactants introduces perturbations of the film thickness over progressively larger distances as the surface elasticity increases. The surface perturbations travel faster than the retracting edge of the film at a speed proportional to the Gibbs modulus. For large values of the Gibbs modulus, the interface behaviour approaches that of an incompressible two-dimensional solid. Our analysis sheds light on the effect of insoluble surfactants on the opening of a circular hole in a thin film and can be extended to investigate the onset of surface cracks and fractures. © 2022 Author

Theory of active self-organization of dense nematic structures in actin gels

The actin cytoskeleton is remarkably adaptable and multifunctional. It often organizes into nematic bundles such as contractile rings or stress fibers. However, how a uniform and isotropic actin gel self-organizes into dense nematic bundles is not understood. Here, using an active gel model accounting for nematic order and density variations, we identify a novel active patterning mechanism leading to dense nematic structures. Linear stability analysis and nonlinear finite element simulations establish the conditions for nematic bundle self-assembly and how active gel parameters control the architecture, orientation, connectivity and dynamics of self-organized patterns. Finally, we substantiate with discrete network simulations the main requirements for nematic bundle formation according to our theory, namely increased active tension perpendicular to the nematic direction and generalized active forces conjugate to nematic order. Our work portrays actin gels a reconfigurable active materials with a spontaneous tendency to develop patterns of dense nematic bundles.Comment: 11 pages, 4 figures, 1 supplementary PDF, 8 supplementary video

Self-Propulsion of Active Colloids via Ion Release: Theory and Experiments

We study the self-propulsion of a charged colloidal particle that releases ionic species using theory and experiments. We relax the assumptions of thin Debye length and weak nonequilibrium effects assumed in classical phoretic models. This leads to a number of unexpected features that cannot be rationalized considering the classic phoretic framework: an active particle can reverse the direction of motion by increasing the rate of ion release and can propel even with zero surface charge. Our theory predicts that there are optimal conditions for self-propulsion and a novel regime in which the velocity is insensitive to the background electrolyte concentration. The theoretical results quantitatively capture the salt-dependent velocity measured in our experiments using active colloids that propel by decomposing urea via a surface enzymatic reaction

Simulaciones numéricas de dinámica de ADN en células

El ADN es un polímero que codifica la información genética de los seres vivos, presente en el núcleo de células eucariotas; en condiciones normales se encuentra compactado en torno a unas estructuras llamadas histonas. Las deformaciones mecánicas de las células y fuerzas externas (principalmente comprimir o estirar el núcleo celular) pueden modificar la conformación del ADN y dar lugar a patologías genéticas, proliferación de cáncer y dañar la información genética. A pesar de su importancia para el correcto funcionamiento y desarrollo de las células, el efecto de fuerzas externas en la organización del ADN es todavía desconocido. En este trabajo se pretende crear un modelo computacional simple para el polímero de ADN, evaluar las fuerzas críticas que el ADN puede soportar antes de perder su conformación así como comprobar si el modelo predice o no que el polímero experimente un comportamiento auxético por aplicación de fuerzas externas de estiramiento, asociado a una descondensación parcial del ADN. <br /

Engineering transient dynamics of artificial cells by stochastic distribution of enzymes

Here the authors develop a coacervate micromotor that can display autonomous motion as a result of stochastic distribution of propelling units. This stochastic-induced mobility is validated and explained through experiments and theory. Random fluctuations are inherent to all complex molecular systems. Although nature has evolved mechanisms to control stochastic events to achieve the desired biological output, reproducing this in synthetic systems represents a significant challenge. Here we present an artificial platform that enables us to exploit stochasticity to direct motile behavior. We found that enzymes, when confined to the fluidic polymer membrane of a core-shell coacervate, were distributed stochastically in time and space. This resulted in a transient, asymmetric configuration of propulsive units, which imparted motility to such coacervates in presence of substrate. This mechanism was confirmed by stochastic modelling and simulations in silico. Furthermore, we showed that a deeper understanding of the mechanism of stochasticity could be utilized to modulate the motion output. Conceptually, this work represents a leap in design philosophy in the construction of synthetic systems with life-like behaviors

The clinical effectiveness of an integrated multidisciplinary evidence-based program to prevent intraoperative pressure injuries in high-risk children undergoing long-duration surgical procedures: a quality improvement study

The prevention of hospital-acquired pressure injuries (HAPIs) in children undergoing long-duration surgical procedures is of critical importance due to the potential for catastrophic sequelae of these generally preventable injuries for the child and their family. Long-duration surgical procedures in children have the potential to result in high rates of HAPI due to physiological factors and the difficulty or impossibility of repositioning these patients intraoperatively. We developed and implemented a multi-modal, multi-disciplinary translational HAPI prevention quality improvement program at a large European Paediatric University Teaching Hospital. The intervention comprised the establishment of wound prevention teams, modified HAPI risk assessment tools, specific education, and the use of prophylactic dressings and fluidized positioners during long-duration surgical procedures. As part of the evaluation of the effectiveness of the program in reducing intraoperative HAPI, we conducted a prospective cohort study of 200 children undergoing long-duration surgical procedures and compared their outcomes with a matched historical cohort of 200 children who had undergone similar surgery the previous year. The findings demonstrated a reduction in HAPI in the intervention cohort of 80% (p &lt; 0.01) compared to the comparator group when controlling for age, pathology, comorbidity, and surgical duration. We believe that the findings demonstrate that it is possible to significantly decrease HAPI incidence in these highly vulnerable children by using an evidence-based, multi-modal, multidisciplinary HAPI prevention strategy

Adherence to antibiotic treatment guidelines and outcomes in the hospitalized elderly with different types of pneumonia

Background: Few studies evaluated the clinical outcomes of Community Acquired Pneumonia (CAP), Hospital-Acquired Pneumonia (HAP) and Health Care-Associated Pneumonia (HCAP) in relation to the adherence of antibiotic treatment to the guidelines of the Infectious Diseases Society of America (IDSA) and the American Thoracic Society (ATS) in hospitalized elderly people (65 years or older). Methods: Data were obtained from REPOSI, a prospective registry held in 87 Italian internal medicine and geriatric wards. Patients with a diagnosis of pneumonia (ICD-9 480-487) or prescribed with an antibiotic for pneumonia as indication were selected. The empirical antibiotic regimen was defined to be adherent to guidelines if concordant with the treatment regimens recommended by IDSA/ATS for CAP, HAP, and HCAP. Outcomes were assessed by logistic regression models. Results: A diagnosis of pneumonia was made in 317 patients. Only 38.8% of them received an empirical antibiotic regimen that was adherent to guidelines. However, no significant association was found between adherence to guidelines and outcomes. Having HAP, older age, and higher CIRS severity index were the main factors associated with in-hospital mortality. Conclusions: The adherence to antibiotic treatment guidelines was poor, particularly for HAP and HCAP, suggesting the need for more adherence to the optimal management of antibiotics in the elderly with pneumonia

Theory and simulations of active and Brownian particles

When in 1827 the botanist Robert Brown was looking through a microscope he recognized particles moving through water in a chaotic way, he was not able to discern the cause of the motion of the particles. Indeed, the criticism he received when trying to publish his results was that he was actually observing `swimming' particles such as bacteria. Both particles moving due to the thermal motion of the fluid molecules and self-propelled organisms `live' in a very particular world, that is the world of Low Reynolds numbers. The physics governing this world, which encompasses both `passive' or `Brownian' particles and `active' or `alive' particles, is extremely different from the physics that governs the macroscopic world, and which we experience every day. At these length-scales, typically of the order of microns, the surface forces (i.e. friction due to the suspending fluid) dominate over the volume forces (i.e. gravity or inertial) and many interesting phenomena arise because of this. Despite being discovered more than a century ago, and being studied for decades, there are many aspects of both Brownian moving particles and of propulsion mechanisms of microorganisms that have to be unveiled yet. The aim of this thesis is to give insights, by means of theoretical analysis and numerical simulations, into two topics that have received much attention in the scientific literature over the past years, namely, the diffusion of particles under confinement and the dynamics of active micro-particles in complex fluids. This thesis is therefore naturally divided in two `macro-sections'; the first one is devoted to the study of Brownian motion of particles under confinement. In the second part of the thesis we present a well known hydrodynamical model to account for self-propulsion of micro-particles such as bacteria or other microorganisms, and we highlight the effects of a complex suspending fluid on the micro-particles dynamics and efficiency